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. 2014 Jan 14;9(1):e85144. doi: 10.1371/journal.pone.0085144

Figure 5. Effects of TGF-β1 on the expression of Cav-1 in human atrial fibroblasts (HAF).

Figure 5

A, Time-dependent alterations of Cav-1 protein levels assessed by Western blotting in HAFs incubated with TGF-β1 of 100 ng/ml for varying periods (0, 6, 12, 24, and 48 h). Note that TGF-β1 induced bi-phasic changes of Cav-1 protein expression with initial upregulation followed by subsequent downregulation. Control HAFs were mock-treated. B, Concentration-dependent effects of TGF-β1 on Cav-1 protein levels assessed by Western blot analysis in HAFs exposed to varying concentrations of TGF-β1 (0, 0.1, 1, 10, 100, and 1000 ng/ml) for 48 h. *P<0.05 vs. Control; n = 3 per group with each measurement conducted in triplicate.