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. 2014 Jan 14;9(1):e85534. doi: 10.1371/journal.pone.0085534

Figure 2. Local treatments induce a systemic syndrome.

Figure 2

(A) A wild-type seedling of P. floridana. (B) Phenotypic variation in a seedling after PfPDS-TRV2 infection for one week. Bars = 5.0 cm. (C) A 3-month old plant infected with PfPDS-TRV2. Bars = 1.5 cm. (D–G) Floral phenotypic variations. In comparison with wild-type floral bud (D) and mature flower (F), the floral bud (E) and mature flower (G) from the PfPDS-TRV2 infected plants are bleached. Bars = 10 mm in D and E, and 25 mm in F and G. (H) ICS from wild-type Physalis. (I) Berry from wild-type Physalis. (J) Mosaic bleached ICS. (K) Completely bleached ICS. (L) Bleached berries with different bleaching degrees from the PfPDS-TRV2 infected plants. Bars = 50 mm in H, J and K, and 25 mm in I and L. (M) Wild-type seeds. (N) Bleached seeds. Bars = 5 mm. (O) Expressions of PfPDS in the PfPDS-TRV2 infected plants. The black column is for the wild type and the other columns are for the PfPDS-TRV2 infected plants with different degrees of bleaching. qRT-PCR was performed using total RNA from the organs indicated. PFACTIN was used as an internal control. The experiments were repeated three times using independent biological samples. Mean expression values and standard deviation are presented.