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. 2014 Jan 14;9(1):e85534. doi: 10.1371/journal.pone.0085534

Figure 4. VIGS-mediated MPF3 silencing phenocopies MPF3-RNAi.

Figure 4

(A) An intact WT small flower bud. (B) An intact WT flower bud. (C) A WT flower. (D) A WT ICS. (E) An intact MPF3-VIGS small flower bud. (F) An intact MPF3-VIGS flower bud. (G) An MPF3-VIGS flower. (H) An MPF3-VIGS ICS. (I) An MPF3-RNAi flower. Bars = 1 mm in A, B, C, E, F, G, and I. Bars = 5 mm in D and H. (J) MPF3 was silenced using a VIGS approach. MPF3 expression was evaluated in five floral organs of VIGS flowers. (K) MPF3 was silenced using an RNAi approach. MPF3 expression was evaluated in five floral organs of RNAi flowers. Total RNA from the indicated mutated floral organs was subjected to qRT-PCR. Gene expression in pedicels of WT samples were set as 1, and PFACTIN was used as an internal control. The dark gray column stands for the gene expression in WT organs light gray column indicates the gene expression in the organs of the mutants, as indicated. The experiments were repeated with three independent biological samples. Mean expression values and standard deviation are presented.