Table 1.

Primer and hydrolysis probe sequences of the SIV and IPC targets.

SIVmac239
Nested Primers for Calibration Curve Construction;
MAC.GAG outer For	5′ – AGGTTACGGCCCGGCGGAAAGAAAA – 3′
MAC.GAG outer Rev	5′ – CCTACTCCCTGACAGGCCGTCAGCATTTCTTC – 3′
MAC.GAG inner For	5′ – AGTACATGTTAAAACATGTAGTATGGGC – 3′
MAC.GAG inner Rev	5′ – CCTTAAGCTTTTGTAGAATCTATCTACATA – 3′
qPCR Primers and Hydrolysis Probe;
SIVmac239 RT Rev	5′ – TATGGGGTTCTGTTGTCT – 3′
SIVmac239 Forward	5′ – AGGCTGCAGATTGGGACTTG – 3′
SIVmac239 Reverse	5′ – TGATCCTGACGGCTCCCTAA – 3′
SIVmac239 Probe	5′ – FAM ACCCACAACCAGCTCCACAACAAGGAC IABKFQ – 3′
SIVagmSAB
Nested Primers for Calibration Curve Construction;
AGM.LTR outer For	5′ – ACTGGGCGGTACTGGGAGTGGCTT – 3′
AGM.LTR outer Rev	5′ – AACTAAGGCAAGACTTTATTGAGG – 3′
AGM.LTR inner For	5′ – ATTGAGCCTGGGTGTTCTCT – 3′
AGM.LTR inner Rev	5′ – CAAGACTTTATTGAGGCAAT – 3′
qPCR Primers and Hydrolysis Probe;
SIVagmSAB Forward	5′ – ATTGAGCCTGGGTGTTCTCT – 3′
SIVagmSAB Reverse	5′ – AAGACCTCACCAGAGTGCTCTTAG – 3′
SIVagmSAB Probe	5′ – FAM TAAGTCTGAACCAGCTTGAGCCTGGGTGTT IABKFQ –
SIVsmE660
Nested Primers for Calibration Curve Construction;
E660.GAG outer For	5′ – TTAGGTTACGGCCCAACGGAAAGA – 3′
E660.GAG outer Rev	5′ – ATACCCAGACCCTTGAGCACCAAT – 3′
E660.GAG inner For	5′ – TTAGGTTACGGCCCAACGGAAAGA – 3′
E660.GAG inner Rev	5′ – GCTGCGGGTGTTGTAAATCCCAAT – 3′
qPCR Primers and Hydrolysis Probe;
SIVsmE660 Forward	5′ – GCAGAGACATCTAGTGGTGGAAAC – 3′
SIVsmE660 Reverse	5′ – GTAATTTCCTCCTCTGCCACTAGG – 3′
SIVsmE660 Probe	5′ – FAM AATGCCAGCAACAAGCAGACCAACAGCA IABKFQ – 3′
HCV-2b (IPC)
qPCR Primers and Hydrolysis Probe;
HCV-2b Forward	5′ – ACACTCCGCCATGAATCACT – 3′
HCV-2b Reverse	5′ – CTGTACGACACTCATACTAACGCC – 3′
HCV-2b probe	5′ – MAX TGTCTTCACGCAGAAAGCGTCTAGCCAT IABKFQ – 3′