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. Author manuscript; available in PMC: 2014 May 18.
Published in final edited form as: Nat Commun. 2013;4:2788. doi: 10.1038/ncomms3788

Figure 5. MEIOB is required for meiosis and fertility in mice of both sexes.

Figure 5

(a) Targeted inactivation of the Meiob gene. The mouse Meiob gene maps to Chromosome 17 and has 14 exons. Deletion of exons 6–10 (aa 111–293) is expected to cause a frame shift in the resulting transcript. (b) Absence of MEIOB protein in P18 Meiob−/− testis. (c) Significant size reduction in 8-wk-old Meiob−/− testis. (d, e) Histological analysis of 8-wk-old wild type and Meiob−/− testes. Abbreviations: Zyg, zygotene spermatocytes; Pa, pachytene spermatocytes; RS, round spermatids; ES, elongated spermatids. (f, g) TUNEL analysis of wild type and Meiob−/− seminiferous tubules. Note the large number of apoptotic cells (green) in Meiob−/− tubules. Scale bar (e, g), 25 µm. (h) Histological analysis of ovaries from adult wild type and Meiob−/− mice. Scale bar, 50 µm. (i–k) Progressive loss of oocytes in Meiob−/− ovaries. Frozen sections prepared from postnatal day 0, 1, and 2 wild type and Meiob−/− ovaries were immunolabeled with anti-YBX2 antibodies. (l) TUNEL analysis of P1 ovaries. Nuclear DNA (i–l) was counterstained with DAPI (blue). Scale bars (i–l), 50 µm.