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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Circulation. 2011 Jul 11;124(4):465–476. doi: 10.1161/CIRCULATIONAHA.110.008698

Figure 1. Cell Free hemoglobin and NO scavenging in stored blood.

Figure 1

(A) Photograph of stored red blood cell supernatant over time. (B) Average cell free plasma heme concentration (μM) over time. (C) Absorbance spectra of representative packed red blood cell supernatants at 4 and 39 days of storage. (D) Concentration (μM) of methemoglobin and oxyhemoglobin by spectral deconvolution of packed red blood cell supernatant. (E) Raw data for NO consumption assay. (F) NO Consumption over Time. (G) Nitric oxide consumption is directly proportional to the plasma heme concentration. (H) Arginase-1 levels increasing over time. Overall P values show are for the significance of the change over time analyzed by RM-ANOVA. For panels (B) and (F) and (H) an * represents a statistically significant difference of time points compared 4 days of storage by Bonferroni correction after RM-ANOVA.