TABLE 3.
Summary of cytokine production of BC3 T cells cultured with Ag and PV-267.
| Ag stimulated cytokine production (pg/ml) |
0 μg/ml PV-267 |
200 μg/ml PV-267 |
Statistical difference *(p value) or NS |
|---|---|---|---|
| IL-8 | 17427.5 ± 10983.3 | 14791.0 ± 7191.8 | NS |
| MIP-1b | 3137.5 ± 2642.7 | 2409.0 ± 652.9 | NS |
| MCP-l(MCAF) | 1701.7 ± 1272.3 | 2001.4 ± 1079.3 | NS |
| IL-6 | 1228.6 ± 729.9 | 738.8 ± 261.0 | NS |
| RANTES | 905.5 ± 350.3 | 1124.9 ± 268.4 | NS |
| IP-10 | 703.8 ± 734.8 | 283.3 ± 302.2 | NS |
| MIP-1a | 399.5 ± 258.8 | 233.2 ± 69.2 | NS |
| PDGF-bb | 312.8 ± 50.5 | 415.9 ± 291.7 | NS |
| TNF-a | 225.8 ± 79.3 | 45.7 ± 34.9 * | p = 0.023 |
| IL-1ra | 215.6 ±27.2 | 128.2 ± 31.5 * | p = 0.022 |
| IL-13 | 126.7 ± 55.5 | 60.1 ± 78.5 | NS |
| VEGF | 96.2 ± 20.4 | 90.7 ± 33.9 | NS |
| IFN-g | 77.5 ± 22.9 | 46.2 ± 17.8 | NS |
| IL-9 | 35.3 ± 9.9 | 27.3 ± 9.1 | NS |
| IL-1b | 30.6 ± 13.2 | 19.7 ± 5.7 | NS |
| IL-10 | 17.9 ± 3.1 | 6.5 ± 2.4 | NS |
| G-CSF | 16.5 ± 9.5 | 11.8 ± 4.3 | NS |
| IL-17 | 15.5 ± 3.6 | 11.0 ± 1.2 | NS |
| Eotaxin | < 16.4 | < 23.5 | NS |
| IL-12(p70) | 9.7 ± 5.4 | 7.2 ± 5.2 | NS |
| FGF | 6.0 ± 1.1 | 2.1 ± 0.6* | p = 0.005 |
| IL-2 | 4.4 ± 0.4 | < 2.7* | p = 0.018 |
| IL-4 | 3.7 ± 0.5 | 2.5 ± 0.9 | NS |
| IL-7 | 2.0 ± 0.4 | < 3.7 | NS |
| IL-5 | < 3.3 | < 0.3 | NS |
| GM-CSF | < 0.5 | < 2.8 | NS |
| IL-15 | < 0.3 | < 0.2 | NS |
MBP specific BC3 T cells were cultured with Ag MBP87-99 or PHA as positive control and autologous APC pretreated with PV-267 at different concentrations for 24 h . Supernatant were collected and analyzed using Bio-Plex assay for human cytokines. Shown are the pooled result of the production of 27 human cytokines when BC3 T cells were cultured with Ag and PV-267 (0 – 200 μg/ml) from at least 3 independent experiments (MEAN±SD). NS, not significant.