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Detection of myelin Ag in CNS-resident microglia in naïve brain. (A–C) Expression of markers CX3CR1 and CD11b in brain tissue sections from naïve CX3CR1+/GFP mice (A) CX3CR1 (green); (B) CD11b (pink); (C) merge A+B. CX3CR1+CD11b+ cells are indicated by arrows. (D–F) CX3CR1+CD11b+ microglia containing MBP (F, small arrows, cells positive for red signal); some of these are also MHCII+ (F, large arrow). Scale bars = 10 μm.
