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. 2014 Jan 14;9(1):e85844. doi: 10.1371/journal.pone.0085844

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© 2014 Basner, Antranikian

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The purification of GDH1E5 was examined after each purification step by SDS-PAGE. Lane 1: Protein standards. Lane 2: Crude extract. Lane 3: Hydrophobic interaction chromatography. Lane 4: Gel filtration. Lane 5: Immobilized metal ion affinity chromatography.