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The influence of di- and trivalent cations on enzyme activity was investigated using D-glucose as substrate and DCPIP as electron acceptor. Residual activity was determined after enzyme preincubation for 30 min in the presence of 1 (black bars) and 5 mM (grey bars) of the corresponding ion in citrate buffer (pH 6.0). Afterwards enzymatic activity was determined in the presence of the cations using DCPIP at 35°C.
