Table 3. Substrate specificity of the GDH1E5.
| Substrate | Specific activity (U/mg)a | Rel. activity (%)b |
| D-glucose | 91.5 (±0.4) | 100.0 |
| D-xylose | 90.8 (±3.3) | 99.2 |
| glucose-6-phosphate | 90.3 (±3.9) | 98.7 |
| D-galactose | 88.0 (±2.4) | 96.2 |
| lactose | 64.5 (±0.6) | 70.5 |
| maltose | 29.7 (±0.6) | 32.5 |
| 2-deoxyglucose | 28.1 (±0.9) | 30.7 |
| fructose-6-phosphate | 10.2 (±0.8) | 11.1 |
| D-mannose | 8.3 (±0.7) | 9.1 |
| sorbitol | 2.6 (±0.4) | 2.8 |
| fructose | 1.0 (±0.1) | 1.1 |
| glucose-1-phosphate | 0.9 (±0.2) | 1.0 |
a All data represent the average of triplicate determinations ± standard derivation using p-nitrosoaniline BM53.0861 as electron acceptor at 35°C and pH 6.0. All substrates were tested at 75 mM concentration.
b rel. activity of all substrates calculated in relation to the activity towards glucose-6-phosphate.