Figure 5. The minimum number of mt protein-coding (PC) genes required to infer the phylogenetic topology of the “supergene” set as a function of taxonomic rank and average % divergence.

A) The number of mt protein-coding (PC) genes minimally required for inferring a statistically indistinuishable topology to that of the “supergene” set (i.e., all 13 mt PC genes) as a function of taxonomic rank of the lineage/dataset. A taxonomic rank of “1” along the x-axis corresponds to a within species phylogeny, “2” corresponds to within genus, etc. Subphylum was the highest rank analyzed, being assigned a taxonomic rank of 33. Taxonomic rank of each lineage followed the convention of NCBI as indicated in Dataset S1. The minimum number of mt protein-coding genes presented here are for phylogenies that utilized amino acid data and were inferred via maximum likelihood. Symbols of larger sizes represent of multiple datasets, with size being porportional to number of datasets. B) The number of mt protein-coding (PC) genes minimally required for inferring a statistically indistinuishable topology to that of the “supergene” set (i.e., all 13 mt PC genes) as a function of average % divergence of the lineage/dataset. The average % divergence was calculated using infoalign in EMBOSS. Minimum number of mt protein-coding genes presented here are for phylogenies that utilized amino acid data and inferred via maximum likelihood.