Fig 1.
The effect of arsenic and TPEN on enhancing UVR-generated 8-OHdG formation. (A) HaCat cells were incubated with the 2μM arsenite and/or 5 μM TPEN for 24h, then exposed to 8 J/cm2 UV radiation on ice. 8-OHdG was measured by immunocytochemistry. (B) The results of (A) were quantified by measuring the optical density of the staining (n ≥ 3). Relative intensity was indicated as the density of treated group divided by that of the control and presented as the means ± SD from three experiments. *, p < 0.01 vs. Control; #, p<0.01 vs. UV alone; &, p<0.05 vs. Arsenic or TPEN alone.