Figure 2.

Time course of HA secretion by undifferentiated GOFs. Serum-starved, hyaluronidase-treated undifferentiated GOFs from 4 strains were cultured with or without IL-1β for 2, 4, and 6 days. Total HA from conditioned media was measured using the modified ELISA. A, HA increased linearly over time in both conditions, and the slope, defined as average HA secretion rate, was greater after IL-1β treatment. The lines were forced through the origin, and error bars indicate SD of data from 4 samples. B, Average secretion rate for individual strains show evidence of strain-to-strain variation.