Figure 4.

SF1 increases prostate cell growth by stimulating cell proliferation. A and B, Ectopic expression of SF1 within BPH-1 cells promotes cell growth. MTT assays comparing cell growth at 24, 48, and 96 hours of culture (A) and quantification of immunocytochemical staining for BrdU incorporation in mCherry (white bars) and mCherrySF1-expressing (dark gray bars) BPH-1 cells (B). C and D, Loss of SF1 expression in BCaPT10 cells slows cell growth. MTT assays (C) and BrdU incorporation quantification (D) performed in shNEG (light gray bars) and shSF1-expressing (black bars) BCaPT10 cells. E, BCaPT10 and BPH-1 cells were treated with vehicle (NTC, white bars), 10 μM abiraterone (ABI, gray bars), or abiraterone plus steroid hormones (black bars, 100 nM DHT, 10 nM estradiol, and 10 nM progestin). B and D results are reported as percentages of positive BrdU-labeled cells per total number of DAPI-stained nuclei. A minimum of 200 cells per condition was used from each of at least 3 independent experiments. Each result is shown as the average ± SEM from at least 3 independent experiments. A–D, Student t test, two-tailed, equal variance. E, One-way ANOVA. *, P < .05; **, P < .01; ***, P < .005; ****, P < .001.