Figure 10.

The clathrin inhibitor pitstop 2 blocks the linear component of membrane retrieval. IHCs in acute explants of P14–P17 organs of Corti were patch clamped in perforated-patch configuration. A, Grand average of depolarization-evoked exocytic ΔC_m (top) and integrated Ca²⁺ influx (bottom) of IHCs treated with 30 μm pitstop 2 (gray) or control cells (black). The numbers at each point indicate the number of cells used for the average. B, C, Grand average of ΔC_m measurements using 20 ms (B) or 200 ms (C) depolarizing stimuli. D, Average time of C_m return to baseline obtained from fitting a linear function to the first 20 s of C_m data after the end of the 20 ms depolarizing pulses summarized in B. E, Average slope of the linear component of exocytosis for 20 ms depolarizing pulses (left, obtained by fitting a linear function to the first 20 s of postdepolarization C_m data) and 200 ms depolarizing pulses (right, obtained by fitting a linear function to the final 5 s of C_m data). F, G, Average amplitudes (F) and time constants (G) of exponential functions fitted to the data summarized in C after subtracting the linear decrease in C_m during the final 5 s of the recording.