Figure 2.

PCB 95-induced synaptogenesis requires RyR activity. DIV6 dissociated hippocampal neurons were transfected with mRFP-tagged β-actin ± empty vector, siRyR1 or siRyR2. On DIV7, cells were pretreated for 1 h with vehicle or FLA365 (10 μm) before treatment with PCB 95 (200 nm) until DIV12. A, Representative photomicrographs of RFP⁺ cells and quantification of dendritic spines and filopodia (n = 23–24 neurons). B–D, Quantitative analyses of mEPSCs (n = 28–32 neurons for control and PCB treatments; n = 11–18 neurons for FLA365 and siRYR treatments) demonstrating PCB 95 effects on mEPSC frequency (B), amplitude (C), and decay time (D) presented as the fold change relative to control neurons. *Significantly different from control at p < 0.01. **Significantly different from control at p < 0.001. ^##Significantly different from PCB 95 alone at p < 0.001 (one-way ANOVA with post hoc Tukey's analysis).