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. 2014 Jan 15;34(3):869–879. doi: 10.1523/JNEUROSCI.4538-13.2014

Figure 5.

Figure 5.

Phosphorylation of GluN2B at Ser1166 is induced by forced swim, but not exposure to fox urine, in vivo. a, Representative Western blots (top) and summary data (bottom) for hippocampal lysates from rats exposed to fox urine for 5 min. Western blots were probed with antibodies to pSer1166-GluN2B, pSer897-GluN1, and pSer845-GluA1 and then with antibodies to total GluN2B, GluN1, and GluA1. Exposure to fox urine increased phosphorylation of GluA1 at Ser845, with little or no effect on phosphorylation levels of GluN2B at Ser1166 or GluN1 at Ser897. n = 4 animals per treatment group, **p = 0.0030. b, Representative Western blots (top) and summary data (bottom) for hippocampal lysates from rats exposed to a single episode of forced swim for 5 min. Western blots were probed with antibodies as above for exposure to fox urine. Forced swim induced striking phosphorylation of GluN2B at Ser1166, GluN1 at Ser897, and GluA1 at Ser845. Values for phosphorylated protein were normalized to the corresponding value for total protein and then to the corresponding value for control animals. n = 5 animals per treatment group. ***p = 0.0006 (S1166 control vs FS). ***p = 0.0002 (S845 control vs FS). ****p < 0.0001.