Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 2;2:2. doi: 10.1186/2051-5960-2-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Feeney et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Second Harmonic Generation (SHG) microscopy of a single fiber from an LOPD muscle biopsy. Stacks of SHG and autofluorescence images were recorded with a step of 0.85 μm to image the whole fiber from top to bottom. SHG (green) shows myosin bands, whereas autofluorescence (red) shows the inclusions. Several images from one series are shown here. The distance from the top of the fiber is indicated. As the focal plane encounters the first inclusions, the interruption of the myosin bands is clear. The interruption becomes the “black hole” of autophagic areas (see text). As the focal plane reaches the other side of the particles, thin partial myosin bands are seen. Arrowheads point to the interruptions, total or partial of the myosin bands; arrows highlight defects in the myofibril alignment. Bar: 25 μm.