Table 1.
Primer sequences used in real-time PCR analysis and ChIP assay
| Target gene | Sequence (F: forward, R: reverse) | GenBank access | Applications |
|---|---|---|---|
| IL-1β |
F: 5′-AACATCGCCACCTACAAG-3′ |
Y15006 |
mRNA quantification |
| R: 5′-TACTCGGTACATACGAGATGGAAA-3′ | |||
| IL-6 |
F: 5′- CATGGACTGGAGCACAAGTA-3′ |
AJ309541.1 |
mRNA quantification |
| R: 5′-TGGAGAGCAGCCCATGTAA-3′ | |||
| FTO |
F: 5′-TGAAGGTAGCGTGGGACATAGA-3′ |
NM_001185147 |
mRNA quantification |
| R: 5′-GGTGAAAAGCCAGCCAGAAC-3′ | |||
| TLR2 |
F:5′-ATCCTGCTGGAGCCCATTCAGAG-3′ |
NM_ 204278.1 |
mRNA quantification |
| R:5′-TTGCTCTTCATCAGGAGGCCACTC -3′ | |||
| TLR4 |
F: 5′-GTTTGACATTGCTCGGTCCT-3′ |
NM_001030693 |
mRNA quantification |
| R: 5′-GCTGCCTCCAGAAGATATGC-3′ | |||
| C/EBPβ |
F: 5′- 5′-ACGAGGCGGACTGTTTGG-3′ |
NM_205253 |
mRNA quantification |
| R: 5′- GCTGCTGGGATGCTGCTAA-3′ | |||
| STAT3 |
F: 5′- CAACAACCCCAAGAAC-3′ |
NM_001030931.1 |
mRNA quantification |
| R: 5′- GCTGAGACCACGCTTT-3′ | |||
| β-Actin |
F: 5′-TGCGTGACATCAAGGAGAAG-3′ |
NM_205518 |
mRNA quantification |
| R: 5′-TGCCAGGGTACATTGTGGTA-3′ | |||
| Fragment 1 |
F: 5′- AGTTTGCCTCATGCTGGTA-3′ |
ENSGALG00000003591 |
ChIP |
| R: 5′- CTGTAGCGAAGTTGGGTGG-3′ | |||
| Fragment 2 |
F: 5′- CCAGGAGTTCCAGCATTA-3′ |
ENSGALG00000003591 |
ChIP |
| R: 5′- ATGGCTCCATCCAGTTGC-3′ |