Table 3.
Methylation levels of EPs according to tissue type
| EP marker |
Probe position |
Placenta (Log
2
) |
Normal blood (Log
2
) |
P value | ||
|---|---|---|---|---|---|---|
| (Start-Stop) | Trisomy 21 | Normal | Pregnant | Non-pregnant | ||
| EP1 |
40357972-40358018 |
0.954 ± 1.087 |
2.495 ± 0.662d,e |
0.000 ± 0.000 |
0.037 ± 0.037 |
0.006 |
| 40358060-40358114 |
0.501 ± 0.678 |
1.663 ± 0.533d,e |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.006 |
|
| 40358069-40358121 |
0.513 ± 0.581 |
1.496 ± 0.468d,e |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.005 |
|
| 40358132-40358191 |
0.172 ± 0.173a |
0.932 ± 0.282d,e |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.000 |
|
| EP2 |
45336733-45336780 |
0.756 ± 1.309 |
1.801 ± 1.081 |
0.000 ± 0.000 |
0.218 ± 0.218 |
0.168 |
| 45336822-45336870 |
0.766 ± 1.327 |
2.247 ± 1.320 |
0.128 ± 0.221 |
0.671 ± 0.131 |
0.143 |
|
| 45336828-45336876 |
0.727 ± 1.259 |
2.151 ± 1.257 |
0.145 ± 0.252 |
0.892 ± 0.106 |
0.151 |
|
| EP3 |
45336822-45336870 |
0.766 ± 1.327 |
2.247 ± 1.320 |
0.263 ± 0.263 |
0.000 ± 0.000 |
0.123 |
| 45336828-45336876 |
0.727 ± 1.259 |
2.151 ± 1.257 |
0.395 ± 0.421 |
0.000 ± 0.000 |
0.145 |
|
| EP4 |
34398855-34398913 |
1.650 ± 1.060 b |
0.833 ± 0.547 |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.045 |
| 34398899-34398948 |
2.423 ± 1.677 b |
1.681 ± 1.018 |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.063 |
|
| EP5 |
43316483-43316539 |
0.775 ± 0.930 |
2.116 ± 0.842 |
0.297 ± 0.515 |
0.685 ± 0.428 |
0.072 |
| 43316494-43316553 |
0.786 ± 0.972 |
2.135 ± 0.914 |
0.207 ± 0.358 |
0.470 ± 0.392 |
0.057 |
|
| 43316641-43316700 |
0.538 ± 0.683 |
0.979 ± 1.097 |
0.446 ± 0.509 |
0.871 ± 0.029 |
0.814 |
|
| EP6 |
43482611-43482655 |
1.159 ± 1.335 |
2.887 ± 1.844 |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.070 |
| 43482725-43482778 |
1.624 ± 0.792b,c |
2.405 ± 0.651d,e |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.001 |
|
| EP7 |
43484105-43484160 |
1.505 ± 0.922b,c |
1.695 ± 0.366d,e |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.001 |
| 43484227-43484272 |
0.430 ± 0.656 |
1.384 ± 0.964d |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.092 |
|
| EP8 |
23481701-23481760 |
0.215 ± 0.372 |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.525 |
| EP9 |
30214808-30214863 |
0.000 ± 0.000 |
0.036 ± 0.062 |
0.000 ± 0.000 |
0.167 ± 0.167 |
0.165 |
| EP10 |
33346983-33347035 |
1.157 ± 0.930 |
1.937 ± 0.338d,e |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.005 |
| 33346985-33347037 |
1.170 ± 1.065 |
1.951 ± 0.301d,e |
0.000 ± 0.000 |
0.000 ± 0.000 |
0.010 |
|
| EP11 |
45254824-45254879 |
0.872 ± 0.896 |
2.002 ± 0.406 |
0.511 ± 0.642 |
1.212 ± 0.173 |
0.088 |
| EP12 | 38919376-38919423 |
0.639 ± 1.106 |
1.762 ± 0.963 |
0.590 ± 0.700 |
1.393 ± 0.050 |
0.361 |
| 38919458-38919517 |
0.814 ± 1.249 |
1.911 ± 0.983 |
0.471 ± 0.651 |
1.412 ± 0.073 |
0.317 |
|
| 38919529-38919577 | 0.812 ± 0.948 | 1.556 ± 0.651 | 0.329 ± 0.570 | 1.122 ± 0.165 | 0.256 | |
Data are presented as mean±standard deviation. Levels were compared using robust one-way analysis of variance (ANOVA), the post hoc Tamhane’s T2 test for multiple comparisons. In post hoc analysis, statistical significance (P < 0.05) is presented as follows: aTrisomy 21 placenta versus normal placenta; bTrisomy 21 placenta versus pregnant women blood; cTrisomy 21 placenta versus non pregnant women blood; dNormal placenta versus pregnant women blood; eNormal placenta versus non pregnant women blood.