Table 1.
Group | IgG (mg/l) | Relative reactivity with lectins (AU) | ||
---|---|---|---|---|
AAL | UEA | LTA | ||
Normal (n = 17) | 67.7 ± 29 | 1.2 ± 0.3 | 0.42 ± 0.2 | 0.35 ± 0.3 |
Leukocytospermia (n = 28) | 131.7 ± 103 | 0.82 ± 0.3 | 0.34 ± 0.3 | 0.51 ± 0.4 |
p < 0.02 | p < 0.002 |
The concentration of IgG in seminal plasmas was determined by radial immunodiffusion according to Mancini et al. [22], using goat anti-human IgG polyclonal antibodies. In both seminal plasma groups, the relative reactivity of IgG (100 ng/100 μl) with fucose-specific lectins was determined by lectin-ELISA [23] using biotinylated lectins AAL, UEA and LTA, and expressed in absorbance units (AU). Results are given as mean value ± standard deviation. A two-tailed p-value of less than 0.05 was considered significant