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. 2013 Sep 22;31(1):51–60. doi: 10.1007/s10719-013-9501-y

Table 1.

Relative reactivity of seminal plasma IgG with fucose-specific lectins

Group IgG (mg/l) Relative reactivity with lectins (AU)
AAL UEA LTA
Normal (n = 17) 67.7 ± 29 1.2 ± 0.3 0.42 ± 0.2 0.35 ± 0.3
Leukocytospermia (n = 28) 131.7 ± 103 0.82 ± 0.3 0.34 ± 0.3 0.51 ± 0.4
p < 0.02 p < 0.002

The concentration of IgG in seminal plasmas was determined by radial immunodiffusion according to Mancini et al. [22], using goat anti-human IgG polyclonal antibodies. In both seminal plasma groups, the relative reactivity of IgG (100 ng/100 μl) with fucose-specific lectins was determined by lectin-ELISA [23] using biotinylated lectins AAL, UEA and LTA, and expressed in absorbance units (AU). Results are given as mean value ± standard deviation. A two-tailed p-value of less than 0.05 was considered significant