Table 2.
Assay performance
|
Analyte |
Plasma |
CSF |
||
|---|---|---|---|---|
| Sensitivity (pg/mL) | Inter-assay CV range (QC concentration range) | Sensitivity (pg/mL) | Inter-assay CV range (QC concentration range) | |
| IL-1Ra |
16 |
8.2 to 3.2 (360 to 10,009 pg/mL) |
Same assay as for plasma |
|
| IL-1α |
6.1 |
9.0 to 4.2 (25 to 366 pg/mL) |
4.75 |
6.6 to 9.2 (20 to 800 pg/mL) |
| IL-1β |
1.3 |
10.3 to 6.3 (17.5 to 146 pg/mL) |
0.48 |
30.3 to 10.9 (1.3 to 10.9 pg/mL) |
| IL-6 |
1.7 |
5.9 to 2 (41 to 322 pg/mL) |
17 |
7.1 to 2.6 (213 to 1,229 pg/mL) |
| IL-8 |
4.7 |
7.9 to 16 (109 to 975 pg/mL) |
17 |
8.9 to 15.8 (185 to 9,152 pg/mL) |
| IL-10 |
1.3 |
10.3 to 6.26 (7.5 to 146 pg/mL) |
0.82 |
10.6 to 5.5 (5.16 to 232 pg/mL) |
| MCP-1 |
45 |
19.5 to 11.3 (207 to 1,759 pg/mL) |
147 |
(19 to 6.5 (9,303 to 11,275 pg/mL) |
| TNF-α |
0.35 |
6.7 to 7.0 (16.8 to 159 pg/mL) |
2.5 |
25 to 15.8 (4.3 to 28.1 pg/mL) |
| CRP | 350 | 14.8 to 1 (9.2 to 97 mg/L) | ND | |
The assay performance data is only for assays used in this study. The lowest sensitivity of the assays is shown and represents either the non-sample value (+2 SD), or the lowest point on the standard curve. Inter-assay CVs are shown from the lowest to the highest concentration of QCs (three or four) that were included in all assays. CRP, C-reactive protein; CV, coefficient of variation; IL, interleukin; IL-1Ra, interleukin-1 receptor antagonist; MCP-1, monocyte chemoattractant protein-1; ND, not determined; QC, quality control; TNF, tumour necrosis factor.