Figure 4.

Bicarbonate ions do not affect the IPSC decay time constant. A, In the presence of bicarbonate ions, contained in normal aCSF, consecutively evoked (gray) and averaged eIPSCs (black) were recorded from cerebellar Purkinje cells with 150 mm internal Cl⁻ solution at −100 mV. B, Evoked IPSCs recorded from the same cell after exchanging aCSF with HCO₃ ⁻-free solution (100% O₂/HEPES buffered). C, Consecutive (gray) and averaged (black) eIPSCs with 10 mm internal Cl⁻ solution at −100 mV. D, Evoked IPSCs recorded from the same cell after exchange with HCO₃ ⁻-free aCSF. E, Cumulative distribution for eIPSC decay time constants at −100 mV with 150 mm (black) and 10 mm (gray) internal Cl⁻ recorded in normal aCSF (dashed lines) and HCO₃ ⁻-free aCSF (solid lines).