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. 2013 Dec 17;3(1):108–115. doi: 10.1242/bio.20136783

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — (A) Time-lapse DIC images of RP-pck2Δ cells forming protrusions in 1 M Sorbitol. P marks appearance of a protrusion. (B) Percentage of cells with the indicated number of protrusions in the indicated conditions. (C) Cell cycle stage of cells initiating a protrusion. Frequency of protrusion appearance at different stages of the cell cycle (I interphase, M mitosis and C cytokinesis). (D) Protrusion volume increase in protruding RP-pck2Δ. Volume increase during growth of walled pck2Δ in 1 M Sorbitol is also shown. (E) Time-lapse DIC images of a protruding RP-pck2Δ cell showing the retraction (R) of the cell body as the protrusion expands. (F) Average protrusion volume in the first video frame where protrusion is visible in RP-pck2Δ cells at the indicated osmolarity. Scale bars: 5 µm.