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. 2014 Jan 15;4:3702. doi: 10.1038/srep03702

Figure 2. DCF1 suppressed glioma cell proliferation, migration and invasion via declining mitochondrial membrane potential and decreaseing ATP synthesis.

Figure 2

(a), (b),The cell proliferation in U251 and U87-MG were significantly inhibited in the expression of DCF1 by CCK8 assay. (c),The migration of U251 was also significantly decreased with DCF1 expression. (d). The schematic diagram of DCF1 deletion in 4 different sites. (e). Full-length sequence of DCF1 leaded to a decrease of cell invasion in U251, but mutants had no effects on it. (f). Full-length sequence of DCF1 leaded to a decrease of mitochondrial membrane potential, but different mutant in DCF1 remained normal state. Scale bars, 200 μm. (g). ATP concentration in cells was significantly reduced compared with mutants in expression of DCF1 suggesting the necessity of a complete sequence of DCF1 in executing its function. (n = 3 per group,*P < 0.05,**P < 0.01,***P < 0.001). Error bars, s.d.