Figure 4. Analyze apoptosis-related proteins and tumorigenicity in vivo.

(a). Western blotting showed that anti-apoptotic protein (Bcl-2) was downregulated, pro-apoptotic protein (Bax) was upregulated, and caspase-3 was activated. (the blots were cropped, and the full-length blots were included in the supplementary information) (b). Prediction of the apoptosis pathway mediated by DCF1. (c), (d). Injection of U251 cells expressed dcf1 into nude mice showed a low tumorigenicity rate and a small tumor volume compared with control groups.