Figure 4.

Recurrent i.p. ovarian cancer model. (A) Intraperitoneal tumors were established with mCherry+ ovarian cancer stem cell (OCSC1-F2) as described in the Material and Methods section. Tumor burden was monitored for 32 days. Data shown are representative of five independent experiments (n = 10). (B) Correlation between mCherry fluorescent signal obtained from live imaging and actual mouse tumor burden. (i) representative image obtained from in vivo FX system 32 days postinjection of cells; (ii) corresponding photograph of carcinomatosis observed postmortem. (C) Treatment with Paclitaxel was initiated and carried out as described in the text. Note that tumors are undetectable in the treated group after four doses of Paclitaxel (Day 11). Recurrence develops in all mice (Day 37). Recurrent disease was not responsive to the second round of Paclitaxel treatment (Day 56). Data shown are representative of five independent experiments (n = 10 animals per group). (D) Treatment with Cisplatin was carried out as described in the text. (i) plot of region of interest (ROI) tumor area from five representative animals treated with Cisplatin; (ii) representative images comparing Control and Cisplatin-treated mouse. Note that in contrast to Paclitaxel, mice progressed with Cisplatin treatment.