(A) Effects of megakaryocytic knockdown of α-actinin-1 on filamin A and Hic-5 expression. Primary human progenitors transduced with shRNA targeting α-actinin-1 (ACTN1) were analyzed as in Figure 5B. (Right panel) Densitometry derived from three independent experiments conducted as in Left panel showing mean ± SEM for relative protein levels normalized to Tubulin. * P < 0.05; ** P < 0.01; NS, not significant. HEXIM1 (H1), α-actinin 1 (Actn1), filamin A (FlnA), tubulin (Tub).
(B–D) Effects of α-actinin-1 knockdown on megakaryocyte morphogenesis. Progenitors transduced with shRNA targeting α-actinin-1 underwent megakaryocyte morphogenesis culture followed by flow cytometry analysis as in Figure 3B–D. Cell morphology was assessed by light microscopy of Wright-stained cytospins (200X). Graphs in (B) show mean ± SEM for three independent experiments. * P < 0.05; NS, not significant. See also
Figure S5.