Skip to main content
. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: Dev Cell. 2013 Dec 23;27(6):607–620. doi: 10.1016/j.devcel.2013.11.013

Figure 6. Contribution of α-actinin-1 to Megakaryocytic Enlargement and Polyploidization.

Figure 6

(A) Effects of megakaryocytic knockdown of α-actinin-1 on filamin A and Hic-5 expression. Primary human progenitors transduced with shRNA targeting α-actinin-1 (ACTN1) were analyzed as in Figure 5B. (Right panel) Densitometry derived from three independent experiments conducted as in Left panel showing mean ± SEM for relative protein levels normalized to Tubulin. * P < 0.05; ** P < 0.01; NS, not significant. HEXIM1 (H1), α-actinin 1 (Actn1), filamin A (FlnA), tubulin (Tub).

(B–D) Effects of α-actinin-1 knockdown on megakaryocyte morphogenesis. Progenitors transduced with shRNA targeting α-actinin-1 underwent megakaryocyte morphogenesis culture followed by flow cytometry analysis as in Figure 3B–D. Cell morphology was assessed by light microscopy of Wright-stained cytospins (200X). Graphs in (B) show mean ± SEM for three independent experiments. * P < 0.05; NS, not significant. See also Figure S5.