Figure 1. Established tissue model for angiogenesis.

(a) The image of cells grew from the mouse lung tissue. Arrows were placed to indicate round shape, “cobblestone” shape and spindle shape cells. Bar = 100 μm. (b) The image of capillary-like structures grew from the lung tissues. Bar = 500 μm. (c) Peripheral cells were identified by transmission electron microscopy. The image showed that the electron micrograph of a weibel-palade body which showed typical longitudinal striations. Bar = 500 nm. (d) Effect of conditioned medium on the TMA. A549 cells cultured supernatant was added to the “sandwich” structure. Addition to the conditioned medium, the number of vessels increased quicker compared with vessels in RPMI-1640 medium group. Values are expressed as means ± SEM. (n = 4). (e) CD34 staining of control. (f) Immunofluorescence micrograph of CD34-stained endothelial cells. The montage image showed the merged fluorescent signal illustrating the human epithelial cells were stained with CD34 marker (green) whereas nuclei were stained with DAPI (blue). Bar = 100 μm. (g) vWF staining of control. (h) Capillary-like structures were stained with vWF-FITC. The representative image showed that the capillary-like structures were labeled with the vWF (green). Bar = 500 μm.