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. 2013 Oct 28;591(Pt 24):6157–6173. doi: 10.1113/jphysiol.2013.263657

Figure 2. Human carotid body stem cell niche.

Figure 2

A, fluorescence immunohistochemistry with antibodies against two stem cell markers, GFAP and nestin, in a thin section of the human carotid body. Scale bar: 10 μm. B, fluorescence immunohistochemistry with antibodies against TH, a glomus cell marker, and against nestin, a stem cell marker, illustrating the presence of neural progenitor cells around a CB glomerulus. Scale bar: 5 μm. C, fluorescence immunohistochemistry image at low magnification with antibodies against DDC, a glomus cell marker, and against nestin, a stem cell marker. Note the proximity between progenitor cells and glomus cells within the CB glomeruli. Scale bar: 20 μm. D, bright-field images of two typical neurospheres obtained in non-adherent cultures after growth of human carotid body stem cells. Scale bar: 100 μm. E, fluorescence immunohistochemistry with antibodies against TH and nestin in thin sections of human carotid body neurospheres, illustrating the presence of nestin+ neural progenitor cells within the core, and blebs of TH+ glomus cells on the surface of neurospheres. Scale bars: 50 μm.