Figure 7. Asynchronous Ca²⁺ waves in PDGFRα⁺ cells.

A, representative time-sequence images of a spontaneous Ca²⁺ wave in a PDGFRα⁺ cell. The duration of the Ca²⁺ wave was 0.56 s. Images were background corrected and the green outlines in each image denote the cell boundary (n= 6, c= 37; scale bar is 10 μm). B-D, Ca²⁺ transients were monitored in PDGFRα⁺ cells before and after ADP. B, Ca²⁺ transients were recorded in arbitrary units from ROIs defined in several PDGFRα⁺ cells (coloured squares). Scale bar is 20 μm. C, cytosolic Ca²⁺ dynamics in 6 PDGFRα⁺ cells shown in coloured ROIs in C under control conditions and after ADP (D). Ca²⁺ transients were stochastic and non-propagating events in PDGFRα⁺ cells before and after stimulation with ADP (n= 6).