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Effects of NIPP-1 and NIPP-2 on procollagen synthesis in LX-2 cells. 10% FBS was used for the tests to eliminate the interference of serum with the produced protein. Procollagen type I C-peptide assay was done after 24 h (A) and 48 h (B) of treatment. Each value was expressed as the mean±SD of triplicate experiments. *P<0.05, **P<0.01 and ***P<0.001 as compared TGF-β1 treated cells. #P<0.001 as compared with TGF-β1 non-treated cells.
