| Reagent | Volume (μL) per sample | Final concentration in 50 μL reaction |
|---|---|---|
| Water | 37.1 – A | |
| Phusion HF buffer (5×) | 10 | 1× |
| dNTPs (25 mM each) | 0.4 | 200 μM each |
| Primer_P1 (10 μM) | 1 | 200 nM |
| Phusion Hot Start High-Fidelity DNA Polymerase (2 U/μL) | 0.5 | 0.02 U/μL |
| Add separately to each well Indexing primer (10 μM) | 1 | 200 nM |
| Template DNA (library) | A |
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