| Reagent | Volume (μL) per sample | Final concentration in 100 μL reaction |
|---|---|---|
| Water | 27 | |
| 5× T4 DNA ligase buffer | 20 | 1× |
| 10 mM ATP | 10 | 1 mM |
| 0 mM dNTP mix | 4 | 0.4 mM |
| T4 DNA polymerase (3 U/μL) | 3 | 0.09 U/μL |
| Klenow DNA polymerase (5 U/μL) | 1 | 0.05 U/μL |
| T4 polynucleotide kinase (10 U/μL) | 5 | 0.5 U/μL |
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