| Reagent | Volume (μL) per sample | Final concentration in 50 μL reaction |
|---|---|---|
| water | 10 | |
| 5× DNA ligase buffer | 10 | 1× |
| Adapter oligo mix | 1 | |
| DNA ligase (1 U/μL) | 5 | 0.1 U/μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.