| Reagent | Volume (μL) per sample | Final concentration in 50 μL reaction |
|---|---|---|
| water | 7 | |
| 5× Phusion-HF Buffer | 10 | 1× |
| PCR primer 1.1 | 1 | |
| PCR primer 2.1 | 1 | |
| 25 mM dNTP mix | 0.5 | 0.25 mM |
| Phusion DNA polymerase | 0.5 | 0.02 U/μL |
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