| Reagent | Volume (μL) | Final concentration in 100 μL reaction |
|---|---|---|
| Hybridization mix for adapter A1 (200 μM): | ||
| Adapter_A1 (500 μM) | 40 | 200 μM |
| Adapter_A3 (500 μM) | 40 | 200 μM |
| Oligo hybridization buffer (10×) | 10 | 1× |
| Water | 10 | |
| Hybridization mix for adapter A2 (200 μM): | ||
| Adapter_A2 (500 μM) | 40 | 200 μM |
| Adapter_ A3 (500 μM) | 40 | 200 μM |
| Oligo hybridization buffer (10×) | 10 | 1× |
| Water | 10 | |
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