| Reagent | Volume (μL) per sample | Final concentration in 40 μL reaction |
|---|---|---|
| Water | 14.1 | |
| ThermoPol reaction buffer (10×) | 4 | 1× |
| dNTPs (25 mM each) | 0.4 | 250 μM each |
| Bst polymerase, large fragment (8 U/μL) | 1.5 | 0.3 U/μL |
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