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. 2011 Sep 7;3(2):e11. doi: 10.4081/idr.2011.e11

Figure 4.

Figure 4

Nanoparticles containing CCR5 and CD4 specific siRNAs inhibit HIV-1 reverse transcription in female reproductive tract explants. Female reproductive tract tissue explants from the endometrium (EM), endocervix (CX) and ectocervix (ECX) were either left untreated (grey bars), or treated with nanoparticles containing irrelevant siRNA (20 nM, white bars), or the combination of both CCR5 and CD4-specific siRNA (10 nM each, black bars) for 48 hrs prior to exposure to HIV-1BaL. Explants were assessed for HIV-1 reverse transcripts 5 days after virus exposure. Data are displayed by setting the levels of HIV-1 DNA from the irrelevant siRNA treated explants to 100%. Data are a compilation from four subjects, each of which was assayed in triplicate. Error bars show the standard deviation of the means relative to the irrelevant siRNA control. The single asterisk (*) denotes statistically significant differences among bracketed bars at the P<0.05 level, and the double asterisks (**) denote significance at the P<0.005 level. Bars with no asterisks did not reach statistical significance at the 95% confidence level.