Figure 5.

Nanoparticles containing siRNA to murine Cd4 reduce Cd4 expression but not Cd45 expression in murine uterine tissues. Nanoparticles containing siRNA to an irrelevant sequence or to murine Cd4 (10 µg/mouse) were instilled directly into each uterine horn. On days 1, 2, 3 and 5, the mice were euthanized and uterine tissues assessed by real-time PCR for Cd4 expression (Panel A) or for Cd45 expression (Panel B) in triplicate and normalized to murine β-actin expression. Average relative expression of Cd4 or Cd45 was compared between groups of mice treated with the irrelevant siRNA control (white bars) or Cd4-specific siRNA (grey bars). Two mice are represented in each group for days one and two and four mice are represented in each group for days 3 and 5. Error bars show the standard deviation of the means among the mice in each group. A statistically significant difference (as denoted by a single asterisk) between the irrelevant and Cd4-specific siRNA treated groups was observed on day 3 (P<0.05). Bars with no asterisk did not reach statistical significance at the 95% confidence level.