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. 2014 Jan 15;4:3701. doi: 10.1038/srep03701

Figure 3. GlcAT-I−/− ESCs fail to differentiate into EBs.

Figure 3

(A), Phase-contrast microscopy of day 4 EBs. (B), Real-time PCR results for pluripotency marker genes, Nanog and Oct4 during in vitro differentiation of ESCs. GAPDH transcripts were used as an internal control. (C), Western blot and quantitative analysis of pluripotency markers, Nanog and Sox2 during in vitro differentiation of ESCs. β-actin was used as a loading control. The gels have been run under the same experimental condition. The blot image was cropped around the region of interest. The results are representative of at least three independent experiments. (D), Real-time PCR results for differentiation marker genes, Gata4, Foxc1, Brachyury, and Nestin during in vitro differentiation of ESCs. GAPDH transcripts were used as an internal control. Statistical analysis was carried out by Student's t test. *P < 0.01 and **P < 0.005.