Table 1. Disaccharide composition of CS from GlcAT-I+/+, GlcAT-I+/−, and GlcAT-I−/− embryonic stem cells. The values are expressed as pmol of disaccharide per mg of dried homogenate of these cells.
pmol/mg (mol%)b | |||
---|---|---|---|
Disaccharidesa | GlcAT-I+/+ | GlcAT-I+/− | GlcAT-I−/− |
ΔDi-0S | 14.2 ± 2.0 (21) | 5.1 ± 2.6 (14) | N.D. |
ΔDi-6S | 6.8 ± 1.8 (10) | 2.0 ± 1.4 (5) | N.D. |
ΔDi-4S | 46.5 ± 4.4 (68) | 29.8 ± 1.0 (80) | N.D. |
ΔDi-diSD | N.D.c | N.D. | N.D. |
ΔDi-diSE | 0.6 ± 0.3 (1) | N.D. | N.D. |
Total (pmol/mg) | 68.1 ± 8.0 | 36.9 ± 5.5 | N.D. |
aAbbreviations: ΔDi-0S, ΔHexUAα1-3GalNAc; ΔDi-6S, ΔHexUAα1-3GalNAc(6-O-sulfate); ΔDi-4S, ΔHexUAα1-3GalNAc(4-O-sulfate); ΔDi-diSD, ΔHexUA(2-O-sulfate)α1-3GalNAc(6-O-sulfate); ΔDi-diSE, ΔHexUAα1-3GalNAc(4,6-O-disulfate).
bThe values are the means from three independent experiments.
cN.D., not detected.