FIG 2 .

Deletion of HVO_2058 affects DolP and Asn-498 glycosylation. (A) In cells lacking HVO_2058, C₅₅ and C₆₀ DolPs are modified by the first three sugars of the complete low-salt tetrasaccharide (left) but not by the complete glycan (right). (B) The S-layer glycoprotein-derived Asn-498-containing peptide from ΔHVO_2058 cells is modified by the first three sugars of the low-salt tetrasaccharide (upper) but not by the complete glycan (lower). (C) In cells of the deletion strain transformed with plasmid pWL-CBD2058, encoding a CBD-tagged version of HVO_2058, the S-layer glycoprotein-derived Asn-498-containing peptide is modified by the complete low-salt tetrasaccharide. The inset shows PCR amplification of CBD-HVO_2058 in the transformed strain but not in the deletion strain. In each panel in the figure, the position of each detected or absent glycan-modified DolP or Asn-498-containing peptide is indicated, as is a schematically drawn depiction of the present or absent glycan. In the schematic drawings, the open circle represents rhamnose, while the filled circles represent hexose.