Figure 3. AMPK associates with BRAF.
(A) FLAG-BRAF proteins coimmunoprecipitate with endogenous AMPKα proteins.
(B) Myc-AMPKα2 proteins coimmunoprecipitate with endogenous BRAF proteins.
(C) Endogenous BRAF coimmunoprecipitate with endogenous AMPKα1 proteins in CCD1106 melanocytes. HEK293 cells were transfected with FLAG-BRAF (A), Myc-AMPKα2 WT, or K45R (kinase-dead, KD) mutant (B) as indicated.