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. 2013 Oct 4;2:204. [Version 1] doi: 10.12688/f1000research.2-204.v1

Figure 2. Characterization of the antibodies by immunofluorescence.

Figure 2.

N2A cells (left columns), or stable N2A transfectants expressing either hMeCP2_e1-myc-his (middle columns) or hMeCP2_e2-myc-his (right columns) were plated on coverslips and labeled with the following antibodies: αe1 (first line), αe2 (second line), Abcam2828 (third line) and αe1+e2 (bottom line), followed by anti-rabbit Alexa633 secondary antibody. Slides were mounted with DAPI-containing medium, and observed with a 40x oil immersion objective. Similar results were obtained in at least five other experiments.