Skip to main content
. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Leukemia. 2012 Dec 18;27(6):1358–1368. doi: 10.1038/leu.2012.366

Figure 5. Silencing nur77 and nor1 suppresses SNDX-275-induced apoptosis.

Figure 5

HL-60 cells (A, B) or primary AML samples (AML10, AML11 and AML12, D–F) were transfected with scrambled siRNA (Scr), siRNA targeting nur77 (SiNur77) or nor1 (SiNor1), or mixture of SiNur77 and SiNor1 (SiMix). Twenty-four hours later, cells were treated with vehicle (0) or 1 μM SNDX-275 for another 24 hours. (A) (i) Nur77 (ii) Nor1 (B) (i) c-Jun and(ii) JunB gene expression in HL-60 cells treated for 24 hr. (C) % of surviving cells (i), and specific apoptosis (ii) was calculated as described in Material and Methods. In (D), (E), and (F), from left to right, the gene expression of Nur77 (i) and Nor1(ii), % surviving cells (iii) and specific apoptosis (iv) are shown for AML10, 11, 12, respectively. The raw data (viable cell numbers and % annexinV(+) cells) are shown in Supplemental Fig. 2Ai, ii for HL-60 and Supplemental Fig. 3A–C for AML1, AML2 and AML3.