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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Leukemia. 2012 Dec 18;27(6):1358–1368. doi: 10.1038/leu.2012.366

Figure 7. SNDX-275 induces the expression of pro-apoptotic proteins Bim and Noxa in AML cells.

Figure 7

(A) Bim (i and ii) and Noxa (iii and iv) gene expression were detected with real-time RT-PCR in AML cell lines (I and iii) and primary AML samples (ii and iv). (B) Bim and Noxa protein expression measured by Western bloting. (C) Caspase 9 cleavage at 24 hr. HL-60 cells and 2 primary AML samples were used to determine the effect of Noxa in SNDX-275-induced apoptosis by SiRNA knockdown. In (D), (E), (F), Noxa gene expression detected by RT-PCR and immunoblotting (i); viable cell numbers (ii) and % apoptotic cells (iii) of HL-60, AML10 and AML11 transfected with siRNA targeting noxa(SiNoxa) is shown.