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Representative binned fluorescence trajectories of donor (green) and acceptor (red) and FRET efficiency trajectories at various GdmCl concentrations. Photons were collected in 1 ms bins at the illumination intensity of 4 kW/cm2. In each trajectory the acceptor bleaches before the donor. The apparent FRET efficiency near zero when the acceptor bleaches indicates that the donor does not have the light-induced red-shifted spectrum [19].
