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. Author manuscript; available in PMC: 2014 Nov 26.
Published in final edited form as: ACS Nano. 2013 Oct 9;7(11):9743–9752. doi: 10.1021/nn403085w

Figure 5.

Figure 5

BMDCs process and display E2-bound SIINFEKL (S) epitopes and activate cognate CD8 T cells. A) MFI measurement of BMDCs showed greater SIINFEKL display when incubated with S-E2 compared to free SIINFEKL peptide at 0.1 µg/ml peptide concentration (** p < 0.01). B) The SIINFEKL-bound E2 group (S-E2) in the B3Z assay showed a 1.7 ± 0.7-fold increase in T cell activation, relative to unbound SIINFEKL. The S-E2 group showed significant B3Z activation above background (DC only), whereas unbound SIINFEKL was statistically within background levels. For both panels A & B, data is presented as mean ± SD (n ≥ 4 independent experiments); ** p < 0.01 and *** p < 0.001, relative to DC only.